Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Clostridium botulinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0172 | - |
VAMP-2 | subtype BoNT/F5, untagged, pH and temperature not specified in the publication | Clostridium botulinum | |
0.0231 | - |
VAMP-2 | His6-tagged subtype BoNT/F7, pH and temperature not specified in the publication | Clostridium botulinum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-dependent protease | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP-25 + H2O | Clostridium botulinum | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
- |
- |
Clostridium botulinum | D2KHS9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Clostridium botulinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP-25 + H2O | - |
Clostridium botulinum | ? | - |
? | |
VAMP-2 + H2O | - |
Clostridium botulinum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * about 100000 + 1 * about 60000, subtype BoNT/F7, SDS-PAGE | Clostridium botulinum |
heterodimer | 1 * about 100000 + 1 * about 70000, subtype BoNT/F5, SDS-PAGE | Clostridium botulinum |
Synonyms | Comment | Organism |
---|---|---|
BoNT/F5 | subtype | Clostridium botulinum |
BoNT/F7 | subtype | Clostridium botulinum |
Botulinum neurotoxin | - |
Clostridium botulinum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
64.69 | - |
VAMP-2 | His6-tagged subtype BoNT/F7, pH and temperature not specified in the publication | Clostridium botulinum | |
594.6 | - |
VAMP-2 | subtype BoNT/F5, untagged, pH and temperature not specified in the publication | Clostridium botulinum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2800 | - |
VAMP-2 | His6-tagged subtype BoNT/F7, pH and temperature not specified in the publication | Clostridium botulinum | |
34570 | - |
VAMP-2 | subtype BoNT/F5, untagged, pH and temperature not specified in the publication | Clostridium botulinum |